PoplarV1, Main, Exploration, bibRecord, 004014

Improved serological diagnosis of Poplar mosaic virus with monoclonal antibodies.

Identifieur interne : 004014 ( Main/Exploration ); précédent : 004013; suivant : 004015

Improved serological diagnosis of Poplar mosaic virus with monoclonal antibodies.

Auteurs : A. Carra [Italie] ; E. Brocchi ; F De Simone ; E. Luisoni

Source :

Journal of virological methods [ 0166-0934 ] ; 2005.

RBID : pubmed:15794987

Descripteurs français

KwdFr :
- Animaux (MeSH), Anticorps monoclonaux (MeSH), Aphides (virologie), Carlavirus (classification), Carlavirus (immunologie), Carlavirus (isolement et purification), Maladies des plantes (virologie), Sensibilité et spécificité (MeSH), Sérums immuns (MeSH), Test ELISA (MeSH).
MESH :
- immunologie : Carlavirus.
- isolement et purification : Carlavirus.
- virologie : Aphides, Maladies des plantes.
- Animaux, Anticorps monoclonaux, Carlavirus, Sensibilité et spécificité, Sérums immuns, Test ELISA.

English descriptors

KwdEn :
- Animals (MeSH), Antibodies, Monoclonal (MeSH), Aphids (virology), Carlavirus (classification), Carlavirus (immunology), Carlavirus (isolation & purification), Enzyme-Linked Immunosorbent Assay (MeSH), Immune Sera (MeSH), Plant Diseases (virology), Sensitivity and Specificity (MeSH).
MESH :
- chemical : Antibodies, Monoclonal, Immune Sera.
- classification : Carlavirus.
- immunology : Carlavirus.
- isolation & purification : Carlavirus.
- virology : Aphids, Plant Diseases.
- Animals, Enzyme-Linked Immunosorbent Assay, Sensitivity and Specificity.

Abstract

Poplar mosaic virus (PopMV) is widespread in all countries where poplar is grown, and causes severe economic losses in terms of quantity and quality of wood production. Control is based on indexing, aimed at the production of healthy propagation material. The currently used diagnostic method is double antibody sandwich (DAS) ELISA with polyclonal antibodies, which is relatively simple and inexpensive and more reliable than visual inspection of symptoms in the nurseries. However, this method also has disadvantages, mainly low sensitivity in relation to low concentration and irregular distribution of the virus in the plant. In this study, a new diagnostic method for PopMV based on production and use of a monoclonal antibody (Mab) in a triple antibody sandwich (TAS) ELISA, is presented. The TAS-ELISA with monoclonal antibodies was optimised by testing a range of reagent combinations and concentrations. PopMV was detected by the optimised TAS-ELISA with sensitivity more than 100 times higher than by DAS-ELISA with polyclonal antibodies. Six PopMV isolates from four European countries were detected with the same efficiency, indicating that no limitations to the practical use of the TAS-ELISA arise due to excessive epitope-specificity of the monoclonal antibody employed.

DOI: 10.1016/j.jviromet.2005.01.014
PubMed: 15794987

Affiliations:

Italie

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Le document en format XML

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<term>Carlavirus (isolation & purification)</term>
<term>Enzyme-Linked Immunosorbent Assay (MeSH)</term>
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<term>Carlavirus (immunologie)</term>
<term>Carlavirus (isolement et purification)</term>
<term>Maladies des plantes (virologie)</term>
<term>Sensibilité et spécificité (MeSH)</term>
<term>Sérums immuns (MeSH)</term>
<term>Test ELISA (MeSH)</term>
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<front><div type="abstract" xml:lang="en">Poplar mosaic virus (PopMV) is widespread in all countries where poplar is grown, and causes severe economic losses in terms of quantity and quality of wood production. Control is based on indexing, aimed at the production of healthy propagation material. The currently used diagnostic method is double antibody sandwich (DAS) ELISA with polyclonal antibodies, which is relatively simple and inexpensive and more reliable than visual inspection of symptoms in the nurseries. However, this method also has disadvantages, mainly low sensitivity in relation to low concentration and irregular distribution of the virus in the plant. In this study, a new diagnostic method for PopMV based on production and use of a monoclonal antibody (Mab) in a triple antibody sandwich (TAS) ELISA, is presented. The TAS-ELISA with monoclonal antibodies was optimised by testing a range of reagent combinations and concentrations. PopMV was detected by the optimised TAS-ELISA with sensitivity more than 100 times higher than by DAS-ELISA with polyclonal antibodies. Six PopMV isolates from four European countries were detected with the same efficiency, indicating that no limitations to the practical use of the TAS-ELISA arise due to excessive epitope-specificity of the monoclonal antibody employed.</div>
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<Abstract><AbstractText>Poplar mosaic virus (PopMV) is widespread in all countries where poplar is grown, and causes severe economic losses in terms of quantity and quality of wood production. Control is based on indexing, aimed at the production of healthy propagation material. The currently used diagnostic method is double antibody sandwich (DAS) ELISA with polyclonal antibodies, which is relatively simple and inexpensive and more reliable than visual inspection of symptoms in the nurseries. However, this method also has disadvantages, mainly low sensitivity in relation to low concentration and irregular distribution of the virus in the plant. In this study, a new diagnostic method for PopMV based on production and use of a monoclonal antibody (Mab) in a triple antibody sandwich (TAS) ELISA, is presented. The TAS-ELISA with monoclonal antibodies was optimised by testing a range of reagent combinations and concentrations. PopMV was detected by the optimised TAS-ELISA with sensitivity more than 100 times higher than by DAS-ELISA with polyclonal antibodies. Six PopMV isolates from four European countries were detected with the same efficiency, indicating that no limitations to the practical use of the TAS-ELISA arise due to excessive epitope-specificity of the monoclonal antibody employed.</AbstractText>
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Improved serological diagnosis of Poplar mosaic virus with monoclonal antibodies.

Improved serological diagnosis of Poplar mosaic virus with monoclonal antibodies.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki